Podocalyxin Like Antibody (2A4) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47750
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat, Rabbit
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Kappa Clone # 2A4
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human Podocalyxin Like (Uniprot: O00592)
Localization
Cell surface and Cytoplasmic
Specificity
Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.
Marker
Hematopoietic Stem Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgM Kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45169).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Podocalyxin Like Antibody (2A4) - Azide and BSA Free
Western Blot: Podocalyxin Like Antibody (2A4)Azide and BSA Free [NBP2-47750]
Western Blot: Podocalyxin Like Antibody (2A4) - Azide and BSA Free [NBP2-47750] - Western Blot Analysis of HeLa cell lysate using Podocalyxin Like Antibody (2A4).Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (2A4) - Azide and BSA Free [NBP2-47750]
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (2A4) - Azide and BSA Free [NBP2-47750] - Human Angiosarcoma stained with Podocalyxin Monoclonal Antibody (2A4).Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (2A4) - Azide and BSA Free [NBP2-47750]
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (2A4) - Azide and BSA Free [NBP2-47750] - Human Placenta stained with Podocalyxin Monoclonal Antibody (2A4).Applications for Podocalyxin Like Antibody (2A4) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
5 - 10 ul/million cells in 0.1ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
1:25 - 1:50
Immunohistochemistry-Paraffin
1:50 - 1:100
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein L or PEG purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Podocalyxin
Alternate Names
GCTM, PCLP, POD XL, PODXL
Gene Symbol
PODXL
Additional Podocalyxin Products
Product Documents for Podocalyxin Like Antibody (2A4) - Azide and BSA Free
Product Specific Notices for Podocalyxin Like Antibody (2A4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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