Podocalyxin Like Antibody (3D3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33108

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Rabbit

Applications

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3D3

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Podocalyxin Antibodies »

Product Specifications

Immunogen

A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human Podocalyxin Like (Uniprot: O00592)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27771514).

Localization

Cell surface and Cytoplasmic

Specificity

Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Marker

Hematopoietic Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-25219).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Podocalyxin Like Antibody (3D3) - Azide and BSA Free

Western Blot: Podocalyxin Like Antibody (3D3)Azide and BSA Free [NBP2-33108]

Western Blot: Podocalyxin Like Antibody (3D3)Azide and BSA Free [NBP2-33108]

Western Blot: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Western Blot Analysis of HeLa cell lysate using Podocalyxin Like Antibody (3D3).
Immunocytochemistry/ Immunofluorescence: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108]

Immunocytochemistry/ Immunofluorescence: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108]

Immunocytochemistry/Immunofluorescence: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Confocal Immunofluorescence of HeLa cells using Podocalyxin Like Antibody (3D3) labeled with CF488 (Green); Red Dot is used to label the nuclei.
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) - Azide and BSA Free [NBP2-33108] - Formalin-paraffin angiosarcoma stained with Podocalyxin Ab (3D3).
View 5 more images

Applications for Podocalyxin Like Antibody (3D3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Frozen

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml

Immunoprecipitation

0.5-1ug/500ug protein lysate

Western Blot

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Podocalyxin

Podocalyxin (PODXL) is a CD34 family member expressed by podocytes, vascular endothelium, mesothelium, and a subset of hematopoietic progenitors. It is the major sialoprotein in the glycocalyx of glomerular podocytes. The physiological role of podocalyxin in leukemic blasts is unknown. However, it is thought of as a candidate prostate cancer tumor aggressiveness gene. Podocalyxin appears to complement CD34 as a useful cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Gene Symbol

PODXL

Additional Podocalyxin Products

Product Documents for Podocalyxin Like Antibody (3D3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Podocalyxin Like Antibody (3D3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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