Podocalyxin Like Antibody [DyLight 405]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-41503V

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Podocalyxin Antibodies »

Product Specifications

Immunogen

A synthetic peptide within an internal region (residues 100-200) of the human Podocalyxin protein. This immunogen should be far enough away from any glycosylation site.

Localization

Membrane

Marker

Podocytes /Apical Marker, Endothelial Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Podocalyxin Like Antibody [DyLight 405]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Podocalyxin

Podocalyxin (PODXL) is an extensively N/O-glycosylated and sialylated type I transmembrane protein that contains C-terminal PDZ-binding motif DTHL capable of facilitating interactions with NHERF1/NHERF2 adaptor proteins which complexes with proteins implicated in ion transport, protein trafficking and signal transduction. PODXL function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in podocytes by charge repulsion. PODXL also acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. It induces apical actin-dependent microvilli formation and is implicated preapical plasma membrane subdomain formation to set up inital epithelial polarization and apical lumen formation during renal tubulogenesis. PODXL is highly expressed in renal glomeruli, embryonic germ layers, hematopoietic progenitors, megakaryocytes and platelets, vascular endothelia, mesothelial cells lining organs, and neurons. In several malignancies such as leukemia and cancer of breast, testicular, prostate etc, PODXL interactions with actin-binding protein EZR enhances cancer aggressiveness by inducing cell migration and invasion through via modulation of MAPK/PI3K signaling.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Gene Symbol

PODXL

Additional Podocalyxin Products

Product Documents for Podocalyxin Like Antibody [DyLight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Podocalyxin Like Antibody [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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