Podocalyxin Like Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13784

Novus Biologicals, part of Bio-Techne
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NBP2-13784
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Podocalyxin Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: QNATQTTTDSSNKTAPTPASSVTIMATDTAQQSTVPTSKANEILASVKATTLGVSSDSPGTTTLAQQVSGPVNTTVARGGGSGNPTTTIESPKSTKSADTTTVATSTATAKPNTTSSQNGAEDTTNSGGKSSHSVTTDLTST

Marker

Podocytes /Apical Marker, Endothelial Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Podocalyxin Like Antibody - BSA Free

Western Blot: Podocalyxin Like Antibody [NBP2-13784]

Western Blot: Podocalyxin Like Antibody [NBP2-13784]

Western Blot: Podocalyxin Like Antibody [NBP2-13784] - Analysis in control (vector only transfected HEK293T lysate) and PODXL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP2-13784]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP2-13784]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP2-13784] - Staining in human kidney and liver tissues using anti-PODXL antibody. Corresponding PODXL RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP2-13784]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP2-13784]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP2-13784] - Staining of human kidney shows high expression.
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Applications for Podocalyxin Like Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Podocalyxin

Podocalyxin (PODXL) is a CD34 family member expressed by podocytes, vascular endothelium, mesothelium, and a subset of hematopoietic progenitors. It is the major sialoprotein in the glycocalyx of glomerular podocytes. The physiological role of podocalyxin in leukemic blasts is unknown. However, it is thought of as a candidate prostate cancer tumor aggressiveness gene. Podocalyxin appears to complement CD34 as a useful cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Gene Symbol

PODXL

Additional Podocalyxin Products

Product Documents for Podocalyxin Like Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Podocalyxin Like Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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