Podocin/NPHS2 Antibody (2O10G3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16111

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16111-100ul
NBP3-16111-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2O10G3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Podocin/NPHS2 Antibodies »

Product Summary for Podocin/NPHS2 Antibody (2O10G3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 284-383 of human Podocin/NPHS2 (Q9NP85). AQRQAKVRMIAAEAEKAASESLRMAAEILSGTPAAVQLRYLHTLQSLSTEKPSTVVLPLPFDLLNCLSSPSNRTQGSLPFPSPSKPVEPLNPKKKDSPML

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Podocin/NPHS2 Antibody (2O10G3)

Western Blot: Podocin/NPHS2 Antibody (2O10G3) [NBP3-16111]

Western Blot: Podocin/NPHS2 Antibody (2O10G3) [NBP3-16111]

Western Blot: Podocin/NPHS2 Antibody (2O10G3) [NBP3-16111] - Western blot analysis of extracts of various cell lines, using Podocin/NPHS2 antibody (NBP3-16111) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Applications for Podocin/NPHS2 Antibody (2O10G3)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Podocin/NPHS2

NPHS2 encodes the glomerular protein podocin which plays a role in the regulation of glomerular permeability, and acts as a linker between the plasma membrane and the cytoskeleton. Defects in this gene are the cause of autosomal recessive steroid-resistant nephrotic syndrome (SRN). SRN is characterized by onset between three months and five years, resistance to steroid therapy and rapid progression to end-stage renal disease. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq]

Alternate Names

Nephrosis 2, NPHS2, PDCN, SRN1

Gene Symbol

NPHS2

Additional Podocin/NPHS2 Products

Product Documents for Podocin/NPHS2 Antibody (2O10G3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Podocin/NPHS2 Antibody (2O10G3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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