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Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-96423

Novus Biologicals, part of Bio-Techne
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NB110-96423SS
NB110-96423

Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # LF3/B7/D5B27

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Membrane protein fraction of isolated rat glomeruli

Marker

Lymphatic Endothelium Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423]

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423]

Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - Non-neoplastic histopathological findings in the abdominal cavity. A: High-power view of anti-podoplanin immunohistochemistry showing single MWCNT A (high dose) nanotubes in the tissue (arrows). B: High-power view of anti-podoplanin immunohistochemistry showing single asbestos fibers in the tissue (arrows). Image collected and cropped by CiteAb from the following publication (https://particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-014-0059-z), licensed under a CC-BY license.
Western Blot: Podoplanin Antibody (LF3/B7/D5B27)Azide and BSA Free [NB110-96423]

Western Blot: Podoplanin Antibody (LF3/B7/D5B27)Azide and BSA Free [NB110-96423]

Western Blot: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - analysis with recombinant human, mouse and rat soluble Podolanin. There is no cross reaction with human and mouse Podoplanin.
Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423]

Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423]

Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - Rat cardiac lymphatic microvessels, labeled with a monoclonal antibody against rat Podoplanin (green) [A] and a polyclonal antibody against mouse LYVE-1 (red) [overlay, B]. Image was obtained at 20x magnification on a Zeiss fluorescence microscope. Scale bar = 50 um. The used protocol in short was: 1. Blockage of nonspecific binding; 2. Incubation with primary abs : anti-mouse Lyve1 (1:1000) and mouse anti- Podoplanin (1:400) for 60 min at RT; 3. Incubation with secondary abs: Donkey anti-rabbit Cy3 and Donkey anti-mouse FITC, 30 min at RT; 4. Mounting in DAPI-containing medium for cell nuclei labeling.

Applications for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

6-30 ug/ml

Immunohistochemistry-Paraffin

6-30 ug/ml

Western Blot

1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Podoplanin

Podoplanin is specifically expressed in the endothelium of lymphatic capillaries but not in the blood vasculature. In normal skin and kidney, podoplanin colocalized with VEGFR3/FLT4, another marker for lymphatic endothelial cells.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Alternate Names

Aggrus, Gp38, PDPN, RANDAM-2, T1A-2

Entrez Gene IDs

54320 (Rat)

Gene Symbol

PDPN

UniProt

Additional Podoplanin Products

Product Documents for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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