Podoplanin Antibody (PDPN/4009R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07494
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Podoplanin Antibody (PDPN/4009R) [NBP3-07494]](https://resources.bio-techne.com/images/products/Podoplanin-Antibody-PDPN-4009R-Immunohistochemistry-Paraffin-NBP3-07494-img0002.jpg "Immunohistochemistry-Paraffin: Podoplanin Antibody (PDPN/4009R) [NBP3-07494]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PDPN/4009R
Concentration
0.2 mg/ml
Product Summary for Podoplanin Antibody (PDPN/4009R)
Immunogen
Synthetic peptide corresponding to residues within aa1-100 of Podoplanin (Uniprot: Q86YL7)
Localization
Cell Surface
Marker
Lymphatic Endothelial & Mesothelial Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08816)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Podoplanin Antibody (PDPN/4009R)
Immunohistochemistry-Paraffin: Podoplanin Antibody (PDPN/4009R) [NBP3-07494]
Immunohistochemistry-Paraffin: Podoplanin Antibody (PDPN/4009R) [NBP3-07494] - Formalin-fixed, paraffin-embedded human tonsil stained with Podoplanin Recombinant Rabbit Monoclonal Antibody (PDPN/4009R).![Immunohistochemistry-Paraffin: Podoplanin Antibody (PDPN/4009R) [NBP3-07494]](https://resources.bio-techne.com/images/products/Podoplanin-Antibody-PDPN-4009R-Immunohistochemistry-Paraffin-NBP3-07494-img0001.jpg "Immunohistochemistry-Paraffin: Podoplanin Antibody (PDPN/4009R) [NBP3-07494]")
Immunohistochemistry-Paraffin: Podoplanin Antibody (PDPN/4009R) [NBP3-07494]
Immunohistochemistry-Paraffin: Podoplanin Antibody (PDPN/4009R) [NBP3-07494] - Formalin-fixed, paraffin-embedded human tonsil stained with Podoplanin Recombinant Rabbit Monoclonal Antibody (PDPN/4009R).
Podoplanin Antibody (PDPN/4009R)
Formalin-fixed, paraffin-embedded human seminoma stained with Podoplanin antibody (PDPN/4009R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for Podoplanin Antibody (PDPN/4009R)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Podoplanin
Long Name
Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A
Alternate Names
Aggrus, Gp38, PDPN, RANDAM-2, T1A-2
Gene Symbol
PDPN
UniProt
Additional Podoplanin Products
Product Documents for Podoplanin Antibody (PDPN/4009R)
Product Specific Notices for Podoplanin Antibody (PDPN/4009R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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