Podoplanin Antibody (PDPN/4009R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07494

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PDPN/4009R

Concentration

0.2 mg/ml

View all Podoplanin Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues within aa1-100 of Podoplanin (Uniprot: Q86YL7)

Localization

Cell Surface

Marker

Lymphatic Endothelial & Mesothelial Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08816)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Podoplanin Antibody (PDPN/4009R)

Immunohistochemistry-Paraffin: Podoplanin Antibody (PDPN/4009R) [NBP3-07494]

Immunohistochemistry-Paraffin: Podoplanin Antibody (PDPN/4009R) [NBP3-07494]

Immunohistochemistry-Paraffin: Podoplanin Antibody (PDPN/4009R) [NBP3-07494] - Formalin-fixed, paraffin-embedded human tonsil stained with Podoplanin Recombinant Rabbit Monoclonal Antibody (PDPN/4009R).
Immunohistochemistry-Paraffin: Podoplanin Antibody (PDPN/4009R) [NBP3-07494]

Immunohistochemistry-Paraffin: Podoplanin Antibody (PDPN/4009R) [NBP3-07494]

Immunohistochemistry-Paraffin: Podoplanin Antibody (PDPN/4009R) [NBP3-07494] - Formalin-fixed, paraffin-embedded human tonsil stained with Podoplanin Recombinant Rabbit Monoclonal Antibody (PDPN/4009R).
Podoplanin Antibody (PDPN/4009R)

Podoplanin Antibody (PDPN/4009R)

Formalin-fixed, paraffin-embedded human seminoma stained with Podoplanin antibody (PDPN/4009R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for Podoplanin Antibody (PDPN/4009R)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Podoplanin

Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a 38 kDa membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Alternate Names

Aggrus, Gp38, PDPN, RANDAM-2, T1A-2

Gene Symbol

PDPN

UniProt

Q86YL7

Additional Podoplanin Products

Product Documents for Podoplanin Antibody (PDPN/4009R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Podoplanin Antibody (PDPN/4009R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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