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Podoplanin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1014

Novus Biologicals, part of Bio-Techne
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NB600-1014-0.025mg
NB600-1014

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Recombinant human Podoplanin

Localization

Type I membrane protein.

Marker

Lymphatic Endothelium Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Podoplanin Antibody

Western Blot: Podoplanin Antibody [NB600-1014]

Western Blot: Podoplanin Antibody [NB600-1014]

Western Blot: Podoplanin Antibody [NB600-1014] - Fig. 1: Western blot analysis of Podoplanin expression in human lymphatic endothelial cells (LEC) and HUVECs. Total lysate of both cell types were subjected to SDS-PAGE and subsequent Western analysis using our monoclonal and polyclonal antibodies. Both antibodies recognize a protein of about 36 kDa in total lysate from LECs but not from HUVEC.

Applications for Podoplanin Antibody

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunoprecipitation

1:10-1:500

Western Blot

1-5 ug/ml
Application Notes
WB: Predicted molecular weight: 24 kDa. Optimal dilutions/concentrations should be determined by the end user. Flow Cytometry reactivity reported in (PMID:21406228).

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Formulation

PBS

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Podoplanin

Podoplanin is specifically expressed in the endothelium of lymphatic capillaries but not in the blood vasculature. In normal skin and kidney, podoplanin colocalized with VEGFR3/FLT4, another marker for lymphatic endothelial cells.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Alternate Names

Aggrus, Gp38, PDPN, RANDAM-2, T1A-2

Entrez Gene IDs

10630 (Human)

Gene Symbol

PDPN

Additional Podoplanin Products

Product Documents for Podoplanin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Podoplanin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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