Podoplanin Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1014
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Recombinant human Podoplanin
Localization
Type I membrane protein.
Marker
Lymphatic Endothelium Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Podoplanin Antibody - Azide and BSA Free
![Western Blot: Podoplanin Antibody [NB600-1014]](https://resources.bio-techne.com/images/products/Podoplanin-Antibody-Western-Blot-NB600-1014-img0001.jpg "Western Blot: Podoplanin Antibody [NB600-1014]")
Western Blot: Podoplanin Antibody [NB600-1014]
Western Blot: Podoplanin Antibody [NB600-1014] - Fig. 1: Western blot analysis of Podoplanin expression in human lymphatic endothelial cells (LEC) and HUVECs. Total lysate of both cell types were subjected to SDS-PAGE and subsequent Western analysis using our monoclonal and polyclonal antibodies. Both antibodies recognize a protein of about 36 kDa in total lysate from LECs but not from HUVEC.Applications for Podoplanin Antibody - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunoprecipitation
1:10-1:500
Western Blot
1-5 ug/ml
Application Notes
WB: Predicted molecular weight: 24 kDa. Optimal dilutions/concentrations should be determined by the end user. Flow Cytometry reactivity reported in (PMID:21406228).
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Podoplanin
Long Name
Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A
Alternate Names
Aggrus, Gp38, PDPN, RANDAM-2, T1A-2
Entrez Gene IDs
10630 (Human)
Gene Symbol
PDPN
Additional Podoplanin Products
Product Documents for Podoplanin Antibody - Azide and BSA Free
Product Specific Notices for Podoplanin Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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