POGZ Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB110-85545
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity purified POGZ Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of mouse POGZ protein. (Uniprot: Q8BZH4)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with POGZ from human, dog, short-tailed opossum, cattle, rat, chimpanzee, macaque, olive baboon, and chicken sources based on 100% homology with the immunizing sequence. Reactivity with POGZ from other sources has not been determined.
Specificity
This antibody is known to react with mouse POGZ protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for POGZ Antibody
![Western Blot: POGZ Antibody [NB110-85545]](https://resources.bio-techne.com/images/products/POGZ-Antibody-Western-Blot-NB110-85545-img0003.jpg "Western Blot: POGZ Antibody [NB110-85545]")
Western Blot: POGZ Antibody [NB110-85545]
Western Blot: POGZ Antibody [NB110-85545] - Analysis of Pogz protein (arrowhead) in adult mouse thymus and spleen tissue lysate (Panel A). The lower molecular weight bands may be cross reactive proteins. Pre-incubation of antibody with the immunizing peptide blocks specific antibody reactivity (Panel B). Primary antibody was used at 1:20,000.Applications for POGZ Antibody
Application
Recommended Usage
ELISA
1:100000-1:400000
Western Blot
1:2000-1:20000
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 155 kDa in size corresponding to Pogz by western blotting in the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: POGZ
Alternate Names
KIAA0461ZNF635, pogo transposable element with ZNF domain, putative protein product of Nbla00003, SUHW5MGC71543, Suppressor of hairy wing homolog 5, Zinc finger protein 280E, Zinc finger protein 635, ZNF280EZNF635m
Gene Symbol
POGZ
UniProt
Additional POGZ Products
Product Documents for POGZ Antibody
Product Specific Notices for POGZ Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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