POLD3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38862

Novus Biologicals, part of Bio-Techne
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NBP2-38862
NBP2-38862-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POLD3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SVTEPKLATPAGLKKSSKKAEPVKVLQKEKKRGKRVALSDDETKETENMRKKRRRIKLPESDSSEDEVFPDSPGAY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLD3 Antibody

Immunocytochemistry/ Immunofluorescence: POLD3 Antibody [NBP2-38862]

Immunocytochemistry/ Immunofluorescence: POLD3 Antibody [NBP2-38862]

Immunocytochemistry/Immunofluorescence: POLD3 Antibody [NBP2-38862] - Staining of human cell line U-251 MG shows localization to nucleus.
Immunohistochemistry-Paraffin: POLD3 Antibody [NBP2-38862]

Immunohistochemistry-Paraffin: POLD3 Antibody [NBP2-38862]

Immunohistochemistry-Paraffin: POLD3 Antibody [NBP2-38862] - Staining in human testis and skeletal muscle tissues using anti-POLD3 antibody. Corresponding POLD3 RNA-seq data are presented for the same tissues.
Immunohistochemistry: POLD3 Antibody [NBP2-38862]

Immunohistochemistry: POLD3 Antibody [NBP2-38862]

Immunohistochemistry: POLD3 Antibody [NBP2-38862] - Staining of human stomach shows strong nuclear positivity in glandular cells.
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Applications for POLD3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLD3

PolD3/p66 is a subunit of the heterotetrameric DNA polymerase delta that plays a role in DNA replication and repair. The PolD3/p66 subunit has been shown to mediate the interaction between DNA polymerase delta and PCNA (proliferating cell nuclear antigen) to facilitate processivity.

Alternate Names

DNA polymerase delta subunit 3, DNA polymerase delta subunit p66, DNA polymerase delta, subunit 3, KIAA0039MGC119643, MGC119642, P66, P68, polymerase (DNA directed), delta 3, polymerase (DNA-directed), delta 3, accessory subunit

Gene Symbol

POLD3

UniProt

Q15054 (Human)

Additional POLD3 Products

Product Documents for POLD3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for POLD3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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