POLDIP2 Antibody (PSH01-20)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32839
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH01-20
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human POLDIP2 aa 31-368 / 368. (Uniprot: Q9Y2S7)
Localization
Mitochondrion matrix, Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for POLDIP2 Antibody (PSH01-20)
Western Blot: POLDIP2 Antibody (PSH01-20) [NBP3-32839]
Western Blot: POLDIP2 Antibody (PSH01-20) [NBP3-32839] - Western blot analysis of POLDIP2 on different lysates with Rabbit anti-POLDIP2 antibody (NBP3-32839) at 1/1,000 dilution.Lane 1: HepG2 cell lysate (20 ug/Lane)
Lane 2: HL-60 cell lysate (20 ug/Lane)
Lane 3: U-87 MG cell lysate (20 ug/Lane)
Lane 4: Rat spleen tissue lysate (40 ug/Lane)
Lane 5: Rat thymus tissue lysate (40 ug/Lane)
Lane 6: Rat kidney tissue lysate (40 ug/Lane)
Lane 7: Rat lung tissue lysate (40 ug/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 8 minutes 54 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32839) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
Applications for POLDIP2 Antibody (PSH01-20)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: POLDIP2
Alternate Names
DKFZP586F1524, p38, PDIP38DKFZp586F1524,38 kDa DNA polymerase delta interaction protein, POLD4, polymerase (DNA-directed), delta interacting protein 2, polymerase delta interacting protein 38, polymerase delta-interacting protein 2
Gene Symbol
POLDIP2
Additional POLDIP2 Products
Product Documents for POLDIP2 Antibody (PSH01-20)
Product Specific Notices for POLDIP2 Antibody (PSH01-20)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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