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POLDIP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-68874

Novus Biologicals, part of Bio-Techne
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NBP1-68874

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence KTHTYYQVLIDARDC corresponding to internal region according to NP_056399.1.

Epitope

KTHTYYQVLIDARDC

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for POLDIP2 Antibody

Western Blot: POLDIP2 Antibody [NBP1-68874]

Western Blot: POLDIP2 Antibody [NBP1-68874]

Western Blot: POLDIP2 Antibody [NBP1-68874] - Staining of K562 (A), MCF7 (B) and Mouse Liver (C) lysates (35ug protein in RIPA buffer). Primary incubation was overnight at 4C. Detected by chemiluminescence.
Western Blot: POLDIP2 Antibody [NBP1-68874]

Western Blot: POLDIP2 Antibody [NBP1-68874]

Western Blot: POLDIP2 Antibody [NBP1-68874] - (1ug/ml) Analysis of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for POLDIP2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 37 kDa band observed in lysates of K562 and MCF7 and of mouse liver (calculated MW of 42.0 kDa band according to NP_056399.1). Approx. 37 kDa band observed in rat vascular smooth muscle cells lysates. The band dimished upon siRNA knock down.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POLDIP2

This gene encodes a protein that interacts with the DNA polymerase delta p50 subunit. The encoded protein also interacts with proliferating cell nuclear antigen. Some transcripts of this gene overlap in a tail-to-tail orientation with the gene for tumor necrosis factor, alpha-induced protein 1 (TNFAIP1).

Alternate Names

DKFZP586F1524, p38, PDIP38DKFZp586F1524,38 kDa DNA polymerase delta interaction protein, POLD4, polymerase (DNA-directed), delta interacting protein 2, polymerase delta interacting protein 38, polymerase delta-interacting protein 2

Entrez Gene IDs

26073 (Human); 67811 (Mouse)

Gene Symbol

POLDIP2

Additional POLDIP2 Products

Product Documents for POLDIP2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for POLDIP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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