POLR2J Antibody (1A10)
Novus Biologicals, part of Bio-Techne | Catalog # H00005439-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1A10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
POLR2J (AAH24165, 1 a.a. ~ 117 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRVAIKDKQEGIE
Specificity
POLR2J - polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa (1A10)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for POLR2J Antibody (1A10)
![Western Blot: POLR2J Antibody (1A10) [H00005439-M02]](https://resources.bio-techne.com/images/products/POLR2J-Antibody-1A10-Western-Blot-H00005439-M02-img0002.jpg "Western Blot: POLR2J Antibody (1A10) [H00005439-M02]")
Western Blot: POLR2J Antibody (1A10) [H00005439-M02]
Western Blot: POLR2J Antibody (1A10) [H00005439-M02] - Analysis of POLR2J expression in transfected 293T cell line by POLR2J monoclonal antibody (M02), clone 1A10.Lane 1: POLR2J transfected lysate(13.3 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: POLR2J Antibody (1A10) [H00005439-M02] - Detection limit for recombinant GST tagged POLR2J is 0.3 ng/ml as a capture antibody.
Applications for POLR2J Antibody (1A10)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: POLR2J
Alternate Names
DNA-directed RNA polymerase II subunit J-1, DNA-directed RNA polymerase II subunit RPB11-a, hRPB14, MGC71910, POLR2J1RNA polymerase II subunit B11-a, polymerase (RNA) II (DNA directed) polypeptide J (13.3kD), polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa, RNA polymerase II 13.3 kDa subunit, RPB11, RPB11a, RPB11m
Entrez Gene IDs
5439 (Human)
Gene Symbol
POLR2J
UniProt
Additional POLR2J Products
Product Documents for POLR2J Antibody (1A10)
Product Specific Notices for POLR2J Antibody (1A10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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