POLR3H Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98184

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all POLR3H Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human POLR3H fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for POLR3H Antibody

Immunocytochemistry/ Immunofluorescence: POLR3H Antibody [NBP2-98184]

Immunocytochemistry/ Immunofluorescence: POLR3H Antibody [NBP2-98184]

Immunocytochemistry/Immunofluorescence: POLR3H Antibody [NBP2-98184] - Immunofluorescence staining of POLR3H in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human POLR3H polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.
Immunohistochemistry-Paraffin: POLR3H Antibody [NBP2-98184]

Immunohistochemistry-Paraffin: POLR3H Antibody [NBP2-98184]

Immunohistochemistry-Paraffin: POLR3H Antibody [NBP2-98184] - Immunochemical staining of human POLR3H in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: POLR3H Antibody [NBP2-98184]

Immunohistochemistry-Paraffin: POLR3H Antibody [NBP2-98184]

Immunohistochemistry-Paraffin: POLR3H Antibody [NBP2-98184] - Immunochemical staining of human POLR3H in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Applications for POLR3H Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLR3H

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleosidetriphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Actsas nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves astemplate for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encodedRNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway

Alternate Names

DNA-directed RNA polymerase III subunit 22.9 kDa polypeptide, DNA-directed RNA polymerase III subunit H, DNA-directed RNA polymerase III subunit RPC8, KIAA1665MGC29654, MGC111097, polymerase (RNA) III (DNA directed) polypeptide H (22.9kD), RNA nucleotidyltransferase (DNA-directed), RNA polymerase III subunit 22.9 kDa subunit, RNA polymerase III subunit C8, RNA polymerase III subunit RPC8, RPC8RPC22.9

Gene Symbol

POLR3H

Additional POLR3H Products

Product Documents for POLR3H Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for POLR3H Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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