PON1 Antibody (OTI2D4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73526

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2D4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PON1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PON1 (NP_000437) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

39.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PON1 Antibody (OTI2D4) - Azide and BSA Free

Western Blot: PON1 Antibody (OTI2D4)Azide and BSA Free [NBP2-73526]

Western Blot: PON1 Antibody (OTI2D4)Azide and BSA Free [NBP2-73526]

Western Blot: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PON1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PON1.
Immunocytochemistry/ Immunofluorescence: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526]

Immunocytochemistry/ Immunofluorescence: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526]

Immunocytochemistry/Immunofluorescence: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PON1.
Immunohistochemistry: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526]

Immunohistochemistry: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526]

Immunohistochemistry: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - Staining of paraffin-embedded Human endometrium tissue using anti-PON1 mouse monoclonal antibody.
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Applications for PON1 Antibody (OTI2D4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PON1

Paraoxonase 1 (PON1) is an arylesterase capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. PON1 primarily hydrolyzes paroxon, an organophosphorus anticholinesterase compound produced in vivo by oxidation of the insecticide parathion, to produce p-nitrophenol. Polymorphisms in this gene are a risk factor in coronary artery disease.

Long Name

Paraoxonase 1

Alternate Names

ESA, K-45

Gene Symbol

PON1

Additional PON1 Products

Product Documents for PON1 Antibody (OTI2D4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PON1 Antibody (OTI2D4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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