Porcine IL-6 Alexa Fluor® 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC6861T
Conjugate
Catalog #
Key Product Details
Species Reactivity
Porcine
Applications
Intracellular Staining by Flow Cytometry
Label
Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 90613
Product Specifications
Immunogen
E. coli-derived recombinant porcine IL-6
Pro29-Met212
Accession # P26893
Pro29-Met212
Accession # P26893
Specificity
Detects porcine IL-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, partial cross‑reactivity with recombinant human, mouse, canine, equine, and feline IL-6 is observed, and no cross‑reactivity with recombinant human (rh) CLC, rhIL-11, rhCNTF, rhCT-1, rhLIF, or rhOSM is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Porcine IL-6 Alexa Fluor® 594-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Sample: Porcine peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
Sample: Porcine peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-6
Interleukin 6 (IL-6) is a pleiotropic alpha-helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa‑28 kDa phosphorylated and variably glycosylated molecule (1‑4). Mature porcine IL-6 is 183 amino acids (aa) in length and shares 61%, 42%, and 42% aa sequence identity with human, mouse, and rat IL-6 (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL‑6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8).
References
- Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.
- Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.
- Jones, S.A. (2005) J. Immunol. 175:3468.
- Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.
- Mathialagan, N. et al. (1992) Mol. Reprod. Dev. 32:324.
- Murakami, M. et al. (1993) Science 260:1808.
- Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
- Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
Long Name
Interleukin 6
Alternate Names
BSF-2, BSF2, IFNB2, IL6, MGI-2A
Entrez Gene IDs
Gene Symbol
IL6
UniProt
Additional IL-6 Products
Product Documents for Porcine IL-6 Alexa Fluor® 594-conjugated Antibody
Product Specific Notices for Porcine IL-6 Alexa Fluor® 594-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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