Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PP2A Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 260 and the C-terminus (residue 309) of human Protein Phosphatase 2A using the numbering given in entry NP_004147.1 (GeneID 5516). The region is identical to that of PPP2CA (NP_00

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PP2A alpha Antibody

Western Blot: PP2A alpha Antibody [NB100-2260]

Western Blot: PP2A alpha Antibody [NB100-2260]

Western Blot: PP2A alpha Antibody [NB100-2260] - Detection of Human and Mouse PP2A by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody NB100-2260 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PP2A alpha Antibody [NB100-2260]

Immunoprecipitation: PP2A alpha Antibody [NB100-2260]

Immunoprecipitation: PP2A alpha Antibody [NB100-2260] - Detection of human PP2A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody NB100-2260 (lot 2) used for IP at 6 ug per reaction. PP2A was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-PP2A antibody NB100-2259. For blotting immunoprecipitated PP2A, NB100-2260 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PP2A alpha Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PP2A

The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B55 subfamily. Defects in the 5' UTR of this gene may cause a rare form of autosomal dominant spinocerebellar ataxia 12.

Alternate Names

PP2CA, PPP2CA, RP-C

Entrez Gene IDs

5515 (Human); 19052 (Mouse)

Gene Symbol

PPP2CA

Additional PP2A Products

Product Documents for PP2A alpha Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PP2A alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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