PP2A alpha/beta Antibody (4J2Z8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16634

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16634-100ul
NBP3-16634-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4J2Z8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PP2A alpha/beta Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-309 of human PP2A alpha/beta (P62714). WSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PP2A alpha/beta Antibody (4J2Z8)

Western Blot: PP2A alpha/beta Antibody (4J2Z8) [NBP3-16634]

Western Blot: PP2A alpha/beta Antibody (4J2Z8) [NBP3-16634]

Western Blot: PP2A alpha/beta Antibody (4J2Z8) [NBP3-16634] - Western blot analysis of extracts of various cell lines, using PP2A alpha/beta Rabbit mAb (NBP3-16634) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: PP2A alpha/beta Antibody (4J2Z8) [NBP3-16634]

Immunocytochemistry/ Immunofluorescence: PP2A alpha/beta Antibody (4J2Z8) [NBP3-16634]

Immunocytochemistry/Immunofluorescence: PP2A alpha/beta Antibody (4J2Z8) [NBP3-16634] - Immunofluorescence analysis of U-2 OS cells using PP2A alpha/beta Rabbit mAb (NBP3-16634) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PP2A alpha/beta Antibody (4J2Z8) [NBP3-16634]

Immunohistochemistry-Paraffin: PP2A alpha/beta Antibody (4J2Z8) [NBP3-16634]

Immunohistochemistry-Paraffin: PP2A alpha/beta Antibody (4J2Z8) [NBP3-16634] - Immunohistochemistry of paraffin-embedded human lung cancer using PP2A alpha/beta Rabbit mAb (NBP3-16634) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for PP2A alpha/beta Antibody (4J2Z8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PP2A alpha/beta

Alternate Names

PPP2CA, PPP2CB, Replication protein C, RP-C, serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, serine/threonine-protein phosphatase 2A catalytic subunit beta isoform

Gene Symbol

PPP2CA

Additional PP2A alpha/beta Products

Product Documents for PP2A alpha/beta Antibody (4J2Z8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PP2A alpha/beta Antibody (4J2Z8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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