PPA2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82180

Novus Biologicals, part of Bio-Techne
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NBP1-82180
NBP1-82180-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PPA2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FALEVIKSTHQCWKALLMKKCNGGAINCTNVQISDSPFRCTQEEARSLVESVSSSPNKESNEEEQVWHFLGK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPA2 Antibody

Western Blot: PPA2 Antibody [NBP1-82180]

Western Blot: PPA2 Antibody [NBP1-82180]

Western Blot: PPA2 Antibody [NBP1-82180] - Analysis using Anti-PPA2 antibody NBP1-82180 (A) shows similar pattern to independent antibody NBP2-38186 (B).
Immunocytochemistry/ Immunofluorescence: PPA2 Antibody [NBP1-82180]

Immunocytochemistry/ Immunofluorescence: PPA2 Antibody [NBP1-82180]

Immunocytochemistry/Immunofluorescence: PPA2 Antibody [NBP1-82180] - Staining of human cell line A-431 shows positivity in mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PPA2 Antibody [NBP1-82180]

Immunohistochemistry-Paraffin: PPA2 Antibody [NBP1-82180]

Immunohistochemistry-Paraffin: PPA2 Antibody [NBP1-82180] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Applications for PPA2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPA2

PPA2 is encoded by this gene is localized to the mitochondrion, is highly similar to members of the inorganic pyrophosphatase (PPase) family, and contains the signature sequence essential for the catalytic activity of PPase. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Alternate Names

EC 3.6.1.1, FLJ20459, MGC49850, mitochondrial, pyrophosphatase (inorganic) 2

Gene Symbol

PPA2

Additional PPA2 Products

Product Documents for PPA2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PPA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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