PPAR delta/NR1C2 Antibody (4E3-1B11)

Novus Biologicals, part of Bio-Techne | Catalog # H00005467-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4E3-1B11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PPAR delta/NR1C2 Antibodies »

Product Specifications

Immunogen

PPARD (AAH02715, 1 a.a. ~ 361 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEQPQEEAPEVREEEEKEEVAEAEGAPELNGGPQHALPSSSYTDLSRSSSPPSLLDQLQMGCDGASCGSLNMECRVCGDKASGFHYGVHACEGCKGFFRRTIRMKLEYEKCERSCKIQKKNRNKCQYCRFQKCLALGMSHNAIRFGRMPEAEKRKLVAGLTANEGSQYNPQVADLKAFSKHIYNAYLKNFNMTKKKARSILTGKASHTAPFVIHDIETLWQAEKGLVWKQLVNGLPPYKEISVHVFYRCQCTTVETVRELTEFAKSIPSFSSLFLNDQVTLLKYGVHEAIFAMLASIVNKDGLLVANGSGFVTREFLRSLRKPFSDIIEPKFEFAVKFNALELDDSDLALFIAAIILCGGE

Specificity

PPARD - peroxisome proliferative activated receptor, delta

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PPAR delta/NR1C2 Antibody (4E3-1B11)

Western Blot: PPAR delta/NR1C2 Antibody (4E3-1B11) [H00005467-M01]

Western Blot: PPAR delta/NR1C2 Antibody (4E3-1B11) [H00005467-M01]

Western Blot: PPAR delta/NR1C2 Antibody (4E3-1B11) [H00005467-M01] - PPARD monoclonal antibody (M01), clone 4E3-1B11 Analysis of PPARD expression in Jurkat.
Proximity Ligation Assay: PPAR delta/NR1C2 Antibody (4E3-1B11) [H00005467-M01]

Proximity Ligation Assay: PPAR delta/NR1C2 Antibody (4E3-1B11) [H00005467-M01]

Proximity Ligation Assay: PPAR delta/NR1C2 Antibody (4E3-1B11) [H00005467-M01] - Analysis of protein-protein interactions between HDAC2 and PPARD. HeLa cells were stained with anti-HDAC2 rabbit purified polyclonal 1:1200 and anti-PPARD mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: PPAR delta/NR1C2 Antibody (4E3-1B11) [H00005467-M01] - Detection limit for recombinant GST tagged PPARD is approximately 0.03ng/ml as a capture antibody.

Applications for PPAR delta/NR1C2 Antibody (4E3-1B11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PPAR delta/NR1C2

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation.

Long Name

Peroxisome Proliferator-activated Receptor delta

Alternate Names

NR1C2, NUC1, PPARD

Entrez Gene IDs

5467 (Human)

Gene Symbol

PPARD

OMIM

600409 (Human)

UniProt

AAH02715 (Human)

Additional PPAR delta/NR1C2 Products

Product Documents for PPAR delta/NR1C2 Antibody (4E3-1B11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PPAR delta/NR1C2 Antibody (4E3-1B11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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