PPAR delta/NR1C2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22468

Novus Biologicals, part of Bio-Techne
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NBP2-22468
NBP2-22468-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Frozen, In vivo assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all PPAR delta/NR1C2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues M(1) E Q P Q E E T P E A R E E(14) C of mouse PPAR delta.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPAR delta/NR1C2 Antibody

Western Blot: PPAR delta/NR1C2 Antibody [NBP2-22468]

Western Blot: PPAR delta/NR1C2 Antibody [NBP2-22468]

Western Blot: PPAR delta/NR1C2 Antibody [NBP2-22468] - Analysis of 25 ug of NIH-3T3 (Lane 1), PC12 (Lane 2), and K562 (Lane 3) cell lysates and a molecular weight protein ladder.
Flow Cytometry: PPAR delta/NR1C2 Antibody [NBP2-22468]

Flow Cytometry: PPAR delta/NR1C2 Antibody [NBP2-22468]

Flow Cytometry: PPAR delta/NR1C2 Antibody [NBP2-22468] - Analysis of PPAR delta was done on jurkat cells. Cells were fixed with 70% ethanol for 10 minutes permeablized with 0.25% Triton (R) X-100 for 20 minutes and blocked with 5% BSA for 30 minutes at room temperature.

Applications for PPAR delta/NR1C2 Antibody

Application
Recommended Usage

Flow Cytometry

3 - 5 ug / 10^6 cells

Immunocytochemistry/ Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:200

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:250 - 1:750
Application Notes
WB: Detects an approx. 49 kDa protein representing PPAR delta from NIH-3T3 cell lysate. Immunolocalization yeilds predominant nuclear staining.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPAR delta/NR1C2

The Peroxisome Proliferator Activated Receptors (PPARs) are members of the nuclear hormone receptor subfamily of transcription factors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. There are 3 known subtypes of PPARs, PPAR alpha, PPAR delta and PPAR gamma. PPAR gamma is believed to be involved in adipocyte differentiation.

Long Name

Peroxisome Proliferator-activated Receptor delta

Alternate Names

NR1C2, NUC1, PPARD

Entrez Gene IDs

25682 (Rat)

Gene Symbol

PPARD

Additional PPAR delta/NR1C2 Products

Product Documents for PPAR delta/NR1C2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PPAR delta/NR1C2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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