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PPAR gamma/NR1C3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-19481

Novus Biologicals, part of Bio-Techne
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NB120-19481

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Hamster

Cited:

Human, Mouse, Rat, Avian - Chicken, Bovine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Synthetic peptide corresponding to the C terminus of human PPAR gamma.

Localization

Nuclear

Specificity

Will recognize both PPARgamma1 and PPARgamma2

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPAR gamma/NR1C3 Antibody

Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481]

Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481]

Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481] - Dnmt3a1 decreases lipid droplet accumulation in the early stage of adipocyte differentiation. Relative mRNA level of some general genes involved in adipogenesis or energy homeostasis after transfection with si-Dnmt3a1 and si-NC into cells for 48 h. Values represent mean +- SEM from four separate experiments. *P less than 0.05, **P less than 0.01, and ***P less than 0.001. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fphys.2018.01270/full) licensed under a CC-BY license.
PPAR gamma/NR1C3 Antibody

Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481] -

Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481] - Dnmt3a1 decreases lipid droplet accumulation in the early stage of adipocyte differentiation. (A) Representative images of oil red O staining (red) & DAPI staining (blue) after transfection with pSDS-Dnmt3a1 & pSDS-NC for 48 h are showed; scale bar: 25 μm. (B) Lipid droplet content by oil red O staining & extraction method of cells transfected with pSDS-Dnmt3a1 & pSDS-NC for 48 h. Values represent mean ± SEM from three independent experiments. ∗∗P < 0.01. (C) Representative images of oil red O staining (red) & DAPI staining (blue) after transfection with si-Dnmt3a1 & si-NC for 48 h are showed; scale bar: 25 μm. (D) Lipid droplet content by oil red O staining & the extraction method of cells transfected with si-Dnmt3a1 & si-NC for 48 h. Values represent mean ± SEM from three independent experiments. ∗P < 0.05. (E) Relative mRNA level of some general genes implicated in adipogenesis or energy homeostasis induced by pSDS-Dnmt3a1 & pSDS-NC into cells. Values represent mean ± SEM from four separate experiments. ∗P < 0.05, ∗∗P < 0.01, & ∗∗∗P < 0.001. (F) Protein expression of some general adipose genes induced by pSDS-Dnmt3a1 & pSDS-NC into cells. (G) Relative mRNA level of some general genes involved in adipogenesis or energy homeostasis after transfection with si-Dnmt3a1 & si-NC into cells for 48 h. Values represent mean ± SEM from four separate experiments. ∗P < 0.05, ∗∗P < 0.01, & ∗∗∗P < 0.001. (H) Protein expression of some general genes involved in adipogenesis at 48 h after transfection by si-Dnmt3a1 & si-NC into cells. The band intensity of Western blotting was obtained by averaging the data from three independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30333755), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PPAR gamma/NR1C3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:400
Application Notes
IHC-P reactivity reported in ( PMID: 25187315).

Reviewed Applications

Read 1 review rated 5 using NB120-19481 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 0.2% gelatin

Preservative

0.1% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PPAR gamma/NR1C3

Peroxisome proliferator-activated receptors (PPAR) are members of the steroid nuclear receptor superfamily (1). The mammalian PPARs include three subtypes PPARalpha, PPARbeta, and PPARgamma. PPARs have been found to regulate fatty acid oxidation, fat cell development, lipoprotein metabolism, and glucose homeostasis (2). Recent studies have found that PPARs can affect the pathogenesis and development of tumors (3). PPARgamma and PPARbeta have been specifically implicated in tumorigenesis (3). PPARalpha is the focus of research studying its function in fatty acid beta-oxidation, lipid metabolism, and vascular inflammation (4). PPARbeta also plays a key role in the activation of keratinocytes during the inflammatory reaction associated with a skin injury (5). Of the three subtypes of PPARs, PPARgamma is the most widely studied. PPARgamma has been implicated in various human chronic diseases such as diabetes mellitus, atherosclerosis, rheumatoid arthritis, inflammatory bowel disease, and Alzheimer's disease (6).

Long Name

Peroxisome Proliferator-activated Receptor gamma

Alternate Names

NR1C3, PPARG

Entrez Gene IDs

5468 (Human); 19016 (Mouse); 25664 (Rat)

Gene Symbol

PPARG

Additional PPAR gamma/NR1C3 Products

Product Documents for PPAR gamma/NR1C3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PPAR gamma/NR1C3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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