PPAR gamma/NR1C3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22106

Novus Biologicals, part of Bio-Techne
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NBP2-22106
NBP2-22106SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, In vivo assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all PPAR gamma/NR1C3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human PPAR gamma protein (between residues 20-120) [UniProt P37231]

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27862692).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PPAR gamma/NR1C3 Antibody - BSA Free

Western Blot: PPAR gamma/NR1C3 AntibodyBSA Free [NBP2-22106]

Western Blot: PPAR gamma/NR1C3 AntibodyBSA Free [NBP2-22106]

Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.
Immunohistochemistry-Paraffin: PPAR gamma/NR1C3 Antibody - BSA Free [NBP2-22106]

Immunohistochemistry-Paraffin: PPAR gamma/NR1C3 Antibody - BSA Free [NBP2-22106]

Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.
Western Blot: PPAR gamma/NR1C3 AntibodyBSA Free [NBP2-22106]

Western Blot: PPAR gamma/NR1C3 AntibodyBSA Free [NBP2-22106]

Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.

Applications for PPAR gamma/NR1C3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

reported in scientific literature (PMID 27862692)

Western Blot

2 ug/ml
Application Notes
In Western blot, a band is detected at ~55 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 2 reviews rated 4 using NBP2-22106 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPAR gamma/NR1C3

Peroxisome proliferator-activated receptor gamma (PPARG), also known as nuclear receptor subfamily 1 group C member 3, NR1C3, GLM1, CIMT1, PPARG1, PPARG2, and PPARgamma, regulates adipocyte differentiation and binds peroxisome proliferators that include hypolipidemic drugs and fatty acids. PPARG regulates the peroxisomal beta-oxidation pathway of these fatty acids. PPARG activates transcription for acyl-CoA oxidase and regulates glucose homeostasis. PPARG is a member of the PPAR family, which is a subfamily of nuclear receptors. PPARG is found in the nucleus and the cytoplasm and is most prevalent in adipose tissue but also found in skeletal muscle, spleen, heart, liver, placenta, lung, and ovary. PPARG is found in three known isoforms (57.6, 54.8, and 21.6 kDa), which are produced by alternative splicing. The PPARG protein is subject to phosphorylation. PPARG has been linked to many diseases such as obesity, diabetes, atherosclerosis, and cancer. Recent research has indicated that mutations in PPARG play a role in non-alcoholic fatty liver disease (PMID: 22160635). Other research has found that using PPARG ligands as part of a chemotherapy treatment could help improve treatment for advanced pancreatic cancer (PMID: 22020928). Similar cancer research discovered that PPARG can be used to induce colorectal carcinoma cell death, making PPARG, in conjunction with survivin and caspase-3, a potential cancer cell regulator (PMID: 21765467).

Long Name

Peroxisome Proliferator-activated Receptor gamma

Alternate Names

NR1C3, PPARG

Entrez Gene IDs

5468 (Human)

Gene Symbol

PPARG

UniProt

P37231 (Human)

Additional PPAR gamma/NR1C3 Products

Product Documents for PPAR gamma/NR1C3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PPAR gamma/NR1C3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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