PPAR gamma/NR1C3 [p Ser273] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44159
Key Product Details
Species Reactivity
Human
Applications
Dot Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PPAR gamma/NR1C3 [p Ser273] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human PPARG protein. (Uniprot: P37231)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with rat, mouse, primate, human, dog, bovine, and pig based on 100% sequence homology. Cross-reactivity with PPAR gamma pS273 from other sources has not been determined.
Modification
p Ser273
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography and is directed against the phosphorylated form of human PPAR gamma/NR1C3 at the Ser273 residue
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for PPAR gamma/NR1C3 [p Ser273] Antibody
![ELISA: PPAR gamma/NR1C3 [p Ser273] Antibody [NBP2-44159]](https://resources.bio-techne.com/images/products/PPAR-gamma-NR1C3-[p-Ser273]-Antibody-ELISA-NBP2-44159-img0004.jpg "ELISA: PPAR gamma/NR1C3 [p Ser273] Antibody [NBP2-44159]")
ELISA: PPAR gamma/NR1C3 [p Ser273] Antibody [NBP2-44159]
ELISA: PPAR gamma/NR1C3 [p Ser273] Antibody [NBP2-44159] - ELISA results of purified Rabbit anti-PPAR gamma pS273 tested against BSA-conjugated non-phospho and phospho forms of immunizing peptide. Each well was coated in duplicate with either 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB substrate.Applications for PPAR gamma/NR1C3 [p Ser273] Antibody
Application
Recommended Usage
ELISA
1:10000
Application Notes
This product has been tested by ELISA and dot blot and is useful for Immunohistochemistry and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~57kDa corresponding to the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: PPAR gamma/NR1C3
Long Name
Peroxisome Proliferator-activated Receptor gamma
Alternate Names
NR1C3, PPARG
Gene Symbol
PPARG
UniProt
Additional PPAR gamma/NR1C3 Products
Product Documents for PPAR gamma/NR1C3 [p Ser273] Antibody
Product Specific Notices for PPAR gamma/NR1C3 [p Ser273] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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