PPAR gamma/NR1C3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-57833

Novus Biologicals, part of Bio-Techne
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NBP2-57833
NBP2-57833-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PPAR gamma/NR1C3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: DYKYDLKLQEYQSAIKVEPASPPYYSEKTQLYNKPHEEPSNSLMAIECRVC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPAR gamma/NR1C3 Antibody

Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-57833]

Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-57833]

Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-57833] - Analysis in human cell lines RT-4 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-57833]

Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-57833]

Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-57833] - Analysis in human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: PPAR gamma/NR1C3 Antibody [NBP2-57833]

Immunocytochemistry/ Immunofluorescence: PPAR gamma/NR1C3 Antibody [NBP2-57833]

Immunocytochemistry/Immunofluorescence: PPAR gamma/NR1C3 Antibody [NBP2-57833] - Staining of human cell line RT4 shows localization to nucleus. Antibody staining is shown in green.

Applications for PPAR gamma/NR1C3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPAR gamma/NR1C3

PPAR gamma(PPARG), which encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

Long Name

Peroxisome Proliferator-activated Receptor gamma

Alternate Names

NR1C3, PPARG

Gene Symbol

PPARG

Additional PPAR gamma/NR1C3 Products

Product Documents for PPAR gamma/NR1C3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PPAR gamma/NR1C3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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