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PPHLN1 Antibody (4B6)

Novus Biologicals, part of Bio-Techne | Catalog # H00051535-M01

Novus Biologicals, part of Bio-Techne
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H00051535-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4B6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PPHLN1 (NP_958846.1, 168 a.a. ~ 258 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KVLDKPSRLTEKELAEAASKWAAEKLEKSDESNLPEISEYEAGSTAPLFTDQPEEPESNTTHGIELFEDSQLTTRSKAIASKTKEIEQVRV

Specificity

PPHLN1 - periphilin 1 (4B6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PPHLN1 Antibody (4B6)

Immunocytochemistry/ Immunofluorescence: PPHLN1 Antibody (4B6) [H00051535-M01]

Immunocytochemistry/ Immunofluorescence: PPHLN1 Antibody (4B6) [H00051535-M01]

Immunocytochemistry/Immunofluorescence: PPHLN1 Antibody (4B6) [H00051535-M01] - Analysis of monoclonal antibody to PPHLN1 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: PPHLN1 Antibody (4B6) [H00051535-M01] - Detection limit for recombinant GST tagged PPHLN1 is 0.3 ng/ml as a capture antibody.

Applications for PPHLN1 Antibody (4B6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PPHLN1

The protein encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Alternate Names

Gastric cancer antigen Ga50, HSPC232, MGC48786, periphilin 1, periphilin-1

Entrez Gene IDs

51535 (Human)

Gene Symbol

PPHLN1

Additional PPHLN1 Products

Product Documents for PPHLN1 Antibody (4B6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PPHLN1 Antibody (4B6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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