PPHLN1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89480

Novus Biologicals, part of Bio-Techne
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NBP1-89480
NBP1-89480-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PPHLN1 Antibodies »

Product Summary for PPHLN1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KKPPLLDRPGEGSYNRYYSHVDYRDYDEGRSFSHDRRSGPPHRGDESGYRWTRDDHSASRQPEYRDMRDGFRRKSFYSSHYARERSPYKRDNTFFRESPVGRKDSPHSR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (83%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPHLN1 Antibody

Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480]

Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480]

Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Analysis in human testis and pancreas tissues. Corresponding PPHLN1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480]

Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480]

Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human cerebral cortex, colon, liver and testis using Anti-MAPRE2 antibody (A) NBP1-89480 shows similar protein distribution across tissues to independent antibody NBP2-38431 (B).
Western Blot: PPHLN1 Antibody [NBP1-89480]

Western Blot: PPHLN1 Antibody [NBP1-89480]

Western Blot: PPHLN1 Antibody [NBP1-89480] - Human foreskin fibroblasts whole cell lysates. Antibody at 1:1000. Image submitted by a verified customer review.
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Applications for PPHLN1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. PPHLN1 antibody validated for WB from a verified customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-89480 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPHLN1

PPHLN1 is encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Alternate Names

Gastric cancer antigen Ga50, HSPC232, MGC48786, periphilin 1, periphilin-1

Gene Symbol

PPHLN1

Additional PPHLN1 Products

Product Documents for PPHLN1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PPHLN1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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