PPHLN1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98653

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PPHLN1 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human PPHLN1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PPHLN1 Antibody

Western Blot: PPHLN1 Antibody [NBP2-98653]

Western Blot: PPHLN1 Antibody [NBP2-98653]

Western Blot: PPHLN1 Antibody [NBP2-98653] - Anti-PPHLN1 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293 Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 53 kDa
Immunocytochemistry/ Immunofluorescence: PPHLN1 Antibody [NBP2-98653]

Immunocytochemistry/ Immunofluorescence: PPHLN1 Antibody [NBP2-98653]

Immunocytochemistry/Immunofluorescence: PPHLN1 Antibody [NBP2-98653] - Immunofluorescence staining of PPHLN1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PPHLN1 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
Immunoprecipitation: PPHLN1 Antibody [NBP2-98653]

Immunoprecipitation: PPHLN1 Antibody [NBP2-98653]

Immunoprecipitation: PPHLN1 Antibody [NBP2-98653] - PPHLN1 was immunoprecipitated using: Lane A: 0.5 mg 293 Whole Cell Lysate 1 ul anti-PPHLN1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PPHLN1 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 55 kDa

Applications for PPHLN1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:2500

Immunoprecipitation

0.2-1uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPHLN1

PPHLN1 is encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Alternate Names

Gastric cancer antigen Ga50, HSPC232, MGC48786, periphilin 1, periphilin-1

Gene Symbol

PPHLN1

Additional PPHLN1 Products

Product Documents for PPHLN1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PPHLN1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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