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PPIG Antibody (4F8)

Novus Biologicals, part of Bio-Techne | Catalog # H00009360-M02

Novus Biologicals, part of Bio-Techne
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H00009360-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4F8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PPIG (NP_004783, 13 a.a. ~ 106 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DIAINNQPAGRVVFELFSDVCPKTCENFRCLCTGEKGTGKSTQKPLHYKSCLFHRVVKDFMVQGGDFSEGNGRGGESIYGGFFEDESFAVKHNK

Reactivity Notes

Human. Other species not tested.

Specificity

PPIG - peptidylprolyl isomerase G (cyclophilin G)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PPIG Antibody (4F8)

Immunocytochemistry/ Immunofluorescence: PPIG Antibody (4F8) [H00009360-M02]

Immunocytochemistry/ Immunofluorescence: PPIG Antibody (4F8) [H00009360-M02]

Immunocytochemistry/Immunofluorescence: PPIG Antibody (4F8) [H00009360-M02] - Analysis of monoclonal antibody to PPIG on HeLa cell . Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: PPIG Antibody (4F8) [H00009360-M02]

Immunohistochemistry-Paraffin: PPIG Antibody (4F8) [H00009360-M02]

Immunohistochemistry-Paraffin: PPIG Antibody (4F8) [H00009360-M02] - Analysis of monoclonal antibody to PPIG on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Sandwich ELISA: PPIG Antibody (4F8) [H00009360-M02] - Detection limit for recombinant GST tagged PPIG is approximately 0.1ng/ml as a capture antibody.

Applications for PPIG Antibody (4F8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PPIG

SR cyclophilin (SRcyp) is also known as peptidyl-prolyl cis-trans isomerase G. Cyclophilins are peptidyl-prolyl isomerases (PPIases) that accelerate the folding of proteins by catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The function of cyclophilins is poorly understood, but their function has been linked to multiple cellular processes such as protein folding, trafficking, and chaperone activity. SRcyp is a member of the Moca family of nuclear cyclophilins and is phosphorylated in a cell cycle dependent manner. There is evidence that SRcyp may be involved in the regulation of gene expression and mRNA splicing. Alternative names for SRcyp include PPIase G, rotamase G, cyclophilin G, clk-associating RS-cyclophilin, CARS-cyclophilin, SR-cyp, CASP10, and PPIG.

Alternate Names

CARS-cyclophilin, CARS-CypPPIase G, CASP10, Clk-associating RS-cyclophilin, Cyclophilin G, CYP, EC 5.2.1.8, MGC133241, peptidyl-prolyl cis-trans isomerase G, Peptidyl-prolyl isomerase G, peptidylprolyl isomerase G (cyclophilin G), peptidyl-prolyl isomerase G (cyclophilin G), Rotamase G, SCAF10, SR-cyclophilin, SRCyp, SR-cyp, SR-related CTD-associated factor 10

Entrez Gene IDs

9360 (Human)

Gene Symbol

PPIG

OMIM

606093 (Human)

Additional PPIG Products

Product Documents for PPIG Antibody (4F8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PPIG Antibody (4F8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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