PPP2R5A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-847

Novus Biologicals, part of Bio-Techne
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NB100-847
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PPP2R5A Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-AYNMHSILSNTSAE corresponding to C-Terminus according to NP_006234.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PPP2R5A Antibody

Western Blot: PPP2R5A Antibody [NB100-847]

Western Blot: PPP2R5A Antibody [NB100-847]

Western Blot: PPP2R5A Antibody [NB100-847] - Staining of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 0.5 ug/mL. Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
Immunohistochemistry-Paraffin: PPP2R5A Antibody [NB100-847]

Immunohistochemistry-Paraffin: PPP2R5A Antibody [NB100-847]

Immunohistochemistry-Paraffin: PPP2R5A Antibody [NB100-847] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: PPP2R5A Antibody [NB100-847]

Immunohistochemistry-Paraffin: PPP2R5A Antibody [NB100-847]

Immunohistochemistry-Paraffin: PPP2R5A Antibody [NB100-847] - Staining of paraffin embedded Human Heart. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for PPP2R5A Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 2 ug/mL
Application Notes
WB: Approx. 55 kDa band observed in human heart and skeletal muscle lysate (calculated MW of 56.2 kDa band according to NP_006234.1). IHC-P: Human heart shows textured cytoplasm staining in cardiomyocytes.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPP2R5A

The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B56 subfamily.

Alternate Names

B56A, MGC131915, PP2A B subunit isoform B56-alpha, PP2A B subunit isoform B'-alpha, PP2A B subunit isoform PR61-alpha, PP2A B subunit isoform R5-alpha, PP2A, B subunit, B' alpha isoform, PP2A, B subunit, B56 alpha isoform, PP2A, B subunit, PR61 alpha isoform, PP2A, B subunit, R5 alpha isoform, PR61A, PR61alpha, protein phosphatase 2, regulatory subunit B (B56), alpha isoform, protein phosphatase 2, regulatory subunit B', alpha, protein phosphatase 2, regulatory subunit B', alpha isoform, serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, alphaisoform, serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform

Entrez Gene IDs

5525 (Human)

Gene Symbol

PPP2R5A

UniProt

Q15172 (Human)

Additional PPP2R5A Products

Product Documents for PPP2R5A Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PPP2R5A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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