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PPT1 Antibody (OTI1F10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45388

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1F10

Concentration

1 mg/ml

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 100-306 of human PPT1 (NP_000301) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

31.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PPT1 Antibody (OTI1F10)

Western Blot: PPT1 Antibody (OTI1F10) [NBP2-45388]

Western Blot: PPT1 Antibody (OTI1F10) [NBP2-45388]

Western Blot: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-PPT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry-Paraffin: PPT1 Antibody (OTI1F10) [NBP2-45388]

Immunohistochemistry-Paraffin: PPT1 Antibody (OTI1F10) [NBP2-45388]

Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: PPT1 Antibody (OTI1F10) [NBP2-45388]

Western Blot: PPT1 Antibody (OTI1F10) [NBP2-45388]

Western Blot: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPT1.

Applications for PPT1 Antibody (OTI1F10)

Application
Recommended Usage

Immunohistochemistry

1:500

Immunohistochemistry-Paraffin

1:500

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPT1

Palmitoyl-protein thioesterase (PPT) is a small glycoprotein that removes palmitate groups from cysteine residues in lipid-modified proteins. PPT is thought to be involved in the catabolism of lipid-modified proteins (Camp et al., 1994 [PubMed 7916016]).[supplied by OMIM]

Alternate Names

ceroid-palmitoyl-palmitoyl-protein thioesterase 1, CLN1EC 3.1.2.22, INCL, Palmitoyl-protein hydrolase 1, palmitoyl-protein thioesterase 1, PPT, PPT-1

Gene Symbol

PPT1

Additional PPT1 Products

Product Documents for PPT1 Antibody (OTI1F10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PPT1 Antibody (OTI1F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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