PRAS40 Antibody (SR1680)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21809

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21809-100ul
NBP3-21809-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1680

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PRAS40 Antibodies »

Product Summary for PRAS40 Antibody (SR1680)

Immunogen

A synthesized peptide derived from human PRAS40 (Uniprot #: Q96B36)

Specificity

Detects endogenous levels of total PRAS40

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PRAS40 Antibody (SR1680)

Western Blot: PRAS40 Antibody (SR1680) [NBP3-21809] -

Western Blot: PRAS40 Antibody (SR1680) [NBP3-21809] - Western blot analysis using NBP3-21809 in (1) HeLa cell lysate; (2) RAW 264.7 cell lysate; (3) PC12 cell lysate.

Applications for PRAS40 Antibody (SR1680)

Application
Recommended Usage

Immunoprecipitation

1:50

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRAS40

Proline Rich Akt Substrate (PRAS40) is a 40 kDa substrate of Akt. Akt and PRAS40 can be found in the phosphoinositide 3 kinase (PI3K) pathway, which plays a role in glucose uptake, cell growth, and apoptosis inhibition. PRAS40 is a 14-3-3 binding protein that reacts with insulin, but whose precise function is not yet known. It may bind SH3 and WW domain containing proteins resulting in a change of function. Activated Akt phosphorylates PRAS40 on threonine 246. Mutation of PRAS40 threonine 246 has been shown to be apoptotic.

Long Name

40 kDa Proline-rich Akt1 Substrate

Alternate Names

AKT1S1, Lobe

Gene Symbol

AKT1S1

Additional PRAS40 Products

Product Documents for PRAS40 Antibody (SR1680)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PRAS40 Antibody (SR1680)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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