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Pref-1/DLK1/FA1 Antibody (PF13-3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80046

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PF13-3

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant human Pref-1/DLK1/FA1 (extracellular domain).

Specificity

Recognizes human Pref-1/DLK1/FA1. Does not cross-react with mouse Pref-1/DLK1/FA1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Pref-1/DLK1/FA1 Antibody (PF13-3) - Azide and BSA Free

Western Blot: Pref-1/DLK1/FA1 Antibody (PF13-3)Azide and BSA Free [NBP2-80046]

Western Blot: Pref-1/DLK1/FA1 Antibody (PF13-3)Azide and BSA Free [NBP2-80046]

Western Blot: Pref-1/DLK1/FA1 Antibody (PF13-3) [NBP2-80046] - Analysis of human DLK-1 [Pref-1] using anti-DLK1 (human), mAb (PF13-3) at 1: 2,000 dilution. Panel A. 1. Human DLK-1-Fc protein. 2. Transfected human DLK-1 full length cell lysate (HEK 293). 3. Mock Transfected HEK293 cell lysate.Panel B. 1. Human DLK-1-Fc protein. 2. Mouse DLK-1-Fc protein. 3. Human DNER-Fc protein. 4. Human DLL1-Fc protein. 5. Human DLL3-Fc protein. 6. Human DLL4-Fc protein. 7. Human Jagged1-Fc protein. 8. Human FGF23-Fc protein.
Immunohistochemistry-Paraffin: Pref-1/DLK1/FA1 Antibody (PF13-3) - Azide and BSA Free [NBP2-80046]

Immunohistochemistry-Paraffin: Pref-1/DLK1/FA1 Antibody (PF13-3) - Azide and BSA Free [NBP2-80046]

Immunohistochemistry-Paraffin: Pref-1/DLK1/FA1 Antibody (PF13-3) [NBP2-80046] - Immunohistochemical staining of DLK1 using anti-DLK1 (human), mAb (PF13-3) in human adrenal tissue (10 ug/mL). This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Pref-1/DLK1/FA1 Antibody (PF13-3) [NBP2-80046] - FACS: DLK1-transfected HEK 293 (Full length) cells were stained significantly using anti-DLK1 (human), mAb (PF13-3).

Applications for Pref-1/DLK1/FA1 Antibody (PF13-3) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:2000 - 1:10000

Flow Cytometry

1:100 - 1:500

Immunohistochemistry

10 ug/mL

Immunohistochemistry-Paraffin

10 ug/mL

Western Blot

1:2000 - 1:10000
Application Notes
Western blot: 1:2000-1:10000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um-filtered solution in PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pref-1/DLK1/FA1

DLK1 may have a role in neuroendocrine differentiation

Long Name

Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1

Alternate Names

DLK-1, DLK1, FA1, pG2, Pref1, ZOG

Gene Symbol

DLK1

Additional Pref-1/DLK1/FA1 Products

Product Documents for Pref-1/DLK1/FA1 Antibody (PF13-3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Pref-1/DLK1/FA1 Antibody (PF13-3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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