PRKD3/nPKC nu Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87948

Novus Biologicals, part of Bio-Techne
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NBP1-87948
NBP1-87948-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PRKD3/nPKC nu Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ANNSPPSAQKSVLPTAIPAVLPAASPCSSPKTGLSARLSNGSFSAPSLTNSRGSVHTVSFLLQIGLTRESVTIEAQELSLSAVKDLVCSIVYQKFPECGFFGM

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRKD3/nPKC nu Antibody

Western Blot: PRKD3/nPKC nu Antibody [NBP1-87948]

Western Blot: PRKD3/nPKC nu Antibody [NBP1-87948]

Western Blot: PRKD3/nPKC nu Antibody [NBP1-87948] - Analysis of C2C12 and UMSCC1 cells with PKD3 antibody (1/1000 dilution) at 4C overnight. Image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: PRKD3/nPKC nu Antibody [NBP1-87948]

Immunocytochemistry/ Immunofluorescence: PRKD3/nPKC nu Antibody [NBP1-87948]

Immunocytochemistry/Immunofluorescence: PRKD3/nPKC nu Antibody [NBP1-87948] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PRKD3/nPKC nu Antibody [NBP1-87948]

Immunohistochemistry-Paraffin: PRKD3/nPKC nu Antibody [NBP1-87948]

Immunohistochemistry-Paraffin: PRKD3/nPKC nu Antibody [NBP1-87948] - Staining of human duodenum shows distinct cytoplasmic positivity in glandular cells.

Applications for PRKD3/nPKC nu Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

PRKD3/nPKC nu antibody validated for WB from a verified customer review.
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 4 using NBP1-87948 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRKD3/nPKC nu

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.

Long Name

Protein Kinase D3

Alternate Names

EPK2, nPKC nu, PKC nu, PRKCN

Gene Symbol

PRKD3

Additional PRKD3/nPKC nu Products

Product Documents for PRKD3/nPKC nu Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PRKD3/nPKC nu Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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