Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3A3

Concentration

0.51 mg/ml

View all PRMT2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PRMT2(NP_001526) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PRMT2 Antibody (OTI3A3)

Western Blot: PRMT2 Antibody (OTI3A3) [NBP2-01291]

Western Blot: PRMT2 Antibody (OTI3A3) [NBP2-01291]

Western Blot: PRMT2 Antibody (3A3) [NBP2-01291] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRMT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRMT2.
Immunocytochemistry/Immunofluorescence: PRMT2 Antibody (3A3) [NBP2-01291] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PRMT2.
Immunohistochemistry-Paraffin: PRMT2 Antibody (OTI3A3) [NBP2-01291]

Immunohistochemistry-Paraffin: PRMT2 Antibody (OTI3A3) [NBP2-01291]

Immunohistochemistry-Paraffin: PRMT2 Antibody (3A3) [NBP2-01291] - Staining of paraffin-embedded Human Kidney tissue using anti-PRMT2 mouse monoclonal antibody.
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Applications for PRMT2 Antibody (OTI3A3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.51 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PRMT2

PRMT2 is a member of the protein arginine N-methyltransferase family that has been shown to be both an androgen receptor (AR)-associated coactivator and an estrogen receptor. PRMT2 expression is upregulated in the lung under hypoxic conditions, and has been shown to inhibit cell activation and promote programmed cell death through a NF-kappaB-dependent mechanism.

Alternate Names

EC 2.1.1.-, EC 2.1.1.125, Histone-arginine N-methyltransferase PRMT2, HMT1, HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 1, HMT1 hnRNP methyltransferase-like 1, HMT1 hnRNP methyltransferase-like 1 (S. cerevisiae), HRMT1L1PRMT2 beta, MGC111373, PRMT2 alpha, PRMT2 gamma, protein arginine methyltransferase 2, protein arginine N-methyltransferase 2

Entrez Gene IDs

3275 (Human)

Gene Symbol

PRMT2

Additional PRMT2 Products

Product Documents for PRMT2 Antibody (OTI3A3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PRMT2 Antibody (OTI3A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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