PRMT6 Antibody (JE36-57)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32854
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE36-57
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human PRMT6 aa 191-240 / 375. (Uniprot: Q96LA8)
Localization
Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PRMT6 Antibody (JE36-57)
Western Blot: PRMT6 Antibody (JE36-57) [NBP3-32854]
Western Blot: PRMT6 Antibody (JE36-57) [NBP3-32854] - Western blot analysis of PRMT6 on different lysates with Rabbit anti-PRMT6 antibody (NBP3-32854) at 1/1,000 dilution.Lane 1: Human kidney tissue lysate (20 ug/Lane)
Lane 2: Mouse kidney tissue lysate (20 ug/Lane)
Lane 3: Mouse testis tissue lysate (20 ug/Lane)
Lane 4: Rat kidney tissue lysate (20 ug/Lane)
Lane 5: Rat testis tissue lysate (20 ug/Lane)
Lane 6: 293T cell lysate (10 ug/Lane)
Lane 7: HEK-293 cell lysate (10 ug/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa (Unknown band detected in mouse tissue)
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32854) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Applications for PRMT6 Antibody (JE36-57)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PRMT6
Long Name
Protein arginine N-methyltransferase 6
Alternate Names
HRMT1L6
Gene Symbol
PRMT6
Additional PRMT6 Products
Product Documents for PRMT6 Antibody (JE36-57)
Product Specific Notices for PRMT6 Antibody (JE36-57)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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