Profilin 1 Antibody (CL3524)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59778

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-59778
NBP2-59778-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL3524

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Profilin 1 Antibodies »

Product Summary for Profilin 1 Antibody (CL3524)

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KSIGGAPTFNVTVTKTDKTLVLLMGKEGIHG

Reactivity Notes

Rat (87%), Mouse (87%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Profilin 1 Antibody (CL3524)

Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778]

Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778]

Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778] - Profilin 1 Antibody (5216) [NBP2-59778] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-PFN1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
Immunohistochemistry-Paraffin: Profilin 1 Antibody (CL3524) [NBP2-59778]

Immunohistochemistry-Paraffin: Profilin 1 Antibody (CL3524) [NBP2-59778]

Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human testis shows weak immunoreactivity in basal cells of the seminiferous ducts.
Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778]

Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778]

Western Blot: Profilin 1 Antibody (5216) [NBP2-59778] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431 and human cell line HepG2.
View 5 more images

Applications for Profilin 1 Antibody (CL3524)

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Profilin 1

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role both in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels. It is also in expressed in skeletal muscle, the uterus and kidneys. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

Alternate Names

PFN1, PROF1, Profilin I

Gene Symbol

PFN1

Additional Profilin 1 Products

Product Documents for Profilin 1 Antibody (CL3524)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Profilin 1 Antibody (CL3524)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.