Profilin 1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19344

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.17 mg/ml

View all Profilin 1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to a C-terminal portion of human Profilin 1 (within residues 100-140). [Swiss-Prot# P07737]

Localization

Cytoplasm, Cytoskeleton.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Profilin 1 Antibody - BSA Free

Western Blot: Profilin 1 AntibodyBSA Free [NBP1-19344]

Western Blot: Profilin 1 AntibodyBSA Free [NBP1-19344]

Western Blot: Profilin 1 Antibody [NBP1-19344] - Western Blot on HeLa whole cell extracts.
Immunohistochemistry: Profilin 1 Antibody - BSA Free [NBP1-19344]

Immunohistochemistry: Profilin 1 Antibody - BSA Free [NBP1-19344]

Immunohistochemistry: Profilin 1 Antibody [NBP1-19344] - Staining of Profilin in mouse thymus.
Simple Western: Profilin 1 AntibodyBSA Free [NBP1-19344]

Simple Western: Profilin 1 AntibodyBSA Free [NBP1-19344]

Simple Western: Profilin 1 Antibody [NBP1-19344] - Simple Western lane view shows a specific band for Profilin 1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for Profilin 1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Simple Western

1:200

Western Blot

0.5 ug/ml
Application Notes
This Profilin 1 antibody is useful for Immunohistochemistry on formalin fixed paraffin embedded sections and Western blot, where a band is seen at approx. 14 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.17 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Profilin 1

Profilin-1 (PFN1) is a widely expressed actin-binding protein that implicates in diverse cellular activities ranging from actin polymerization regulation to control of gene transcription. PFN1 facilitate actin polymerization and cell movement by catalyzing the exchange of actin-bound ADP for ATP, and by liberating actin monomer (G-actin) from its sequestering protein, thymosin-beta4; both processes leading to unidirectional addition of G-actin to elongating actin filaments. PFN1 binds actin for impacting cytoskeletal structures, and interestingly, at high concentrations, PFN1 prevents polymerization of actin, and vice versa. PFN1-PIP2 binding inhibits the formation of IP3 and DG, and PFN1 also inhibits AR/HTT aggregation via G-actin binding. Indeed, PFN1 phosphorylation at Ser-138 site has been shown to reduce its affinity for G-actin that follows inhibition of PFN1/HTT interactions and AR/HTT aggregation. PFN1 has long been considered an essential control element for actin polymerization and cell migration, however, recent data suggests an inhibitory action of PFN1 on motility of certain types of carcinoma cells. PFN1 gene disruption causes gross impairment of development and embryonic lethality in insects/mice models abd defects in PFN1 have been linked to amyotrophic lateral sclerosis 18 (ALS18)

Alternate Names

PFN1, PROF1, Profilin I

Gene Symbol

PFN1

Additional Profilin 1 Products

Product Documents for Profilin 1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Profilin 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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