Profilin 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99325

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Profilin 1 Antibodies »

Product Summary for Profilin 1 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Profilin 1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Profilin 1 Antibody

Western Blot: Profilin 1 Antibody [NBP2-99325]

Western Blot: Profilin 1 Antibody [NBP2-99325]

Western Blot: Profilin 1 Antibody [NBP2-99325] - Lane A: HeLa Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa
Immunocytochemistry/ Immunofluorescence: Profilin 1 Antibody [NBP2-99325]

Immunocytochemistry/ Immunofluorescence: Profilin 1 Antibody [NBP2-99325]

Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody [NBP2-99325] - Immunofluorescence staining of Profilin 1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Profilin 1 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to cytoplasm.

Applications for Profilin 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Profilin 1

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role both in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels. It is also in expressed in skeletal muscle, the uterus and kidneys. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

Alternate Names

PFN1, PROF1, Profilin I

Gene Symbol

PFN1

Additional Profilin 1 Products

Product Documents for Profilin 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Profilin 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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