Progesterone R/NR3C3 Antibody (OTI11E8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73607

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI11E8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Progesterone R/NR3C3 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-298 of human PGR (NP_000917) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

98.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Progesterone R/NR3C3 Antibody (OTI11E8) - Azide and BSA Free

Western Blot: Progesterone R/NR3C3 Antibody (OTI11E8)Azide and BSA Free [NBP2-73607]

Western Blot: Progesterone R/NR3C3 Antibody (OTI11E8)Azide and BSA Free [NBP2-73607]

Western Blot: Progesterone R/NR3C3 Antibody (OTI11E8) - Azide and BSA Free [NBP2-73607] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Progesterone R.
Immunohistochemistry: Progesterone R/NR3C3 Antibody (OTI11E8) - Azide and BSA Free [NBP2-73607]

Immunohistochemistry: Progesterone R/NR3C3 Antibody (OTI11E8) - Azide and BSA Free [NBP2-73607]

Immunohistochemistry: Progesterone R/NR3C3 Antibody (OTI11E8) - Azide and BSA Free [NBP2-73607] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Flow Cytometry: Progesterone R/NR3C3 Antibody (OTI11E8) - Azide and BSA Free [NBP2-73607]

Flow Cytometry: Progesterone R/NR3C3 Antibody (OTI11E8) - Azide and BSA Free [NBP2-73607]

Flow Cytometry: Progesterone R/NR3C3 Antibody (OTI11E8) - Azide and BSA Free [NBP2-73607] - Analysis of MCF-7 cells penetrated, using PGR antibody,(Red), compared to a nonspecific negative control antibody (Blue). Dilution: 1:200
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Applications for Progesterone R/NR3C3 Antibody (OTI11E8) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Progesterone R/NR3C3

The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.

Long Name

Progesterone Receptor

Alternate Names

NR3C3, PGR, ProgesteroneR

Gene Symbol

PGR

Additional Progesterone R/NR3C3 Products

Product Documents for Progesterone R/NR3C3 Antibody (OTI11E8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Progesterone R/NR3C3 Antibody (OTI11E8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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