Prohibitin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19944

Novus Biologicals, part of Bio-Techne
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NBP2-19944
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (96%), Feline (100%), Primate (100%), Rhesus Macaque (100%), Zebrafish (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Prohibitin Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Prohibitin. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (100%), Cat (100%), Xenopus laevis (89%).

Localization

Mitochondrion inner membrane

Marker

Mitochondria Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Prohibitin Antibody

Western Blot: Prohibitin Antibody [NBP2-19944]

Western Blot: Prohibitin Antibody [NBP2-19944]

Western Blot: Prohibitin Antibody [NBP2-19944] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract12 % SDS-PAGE Prohibitin antibody dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: Prohibitin Antibody [NBP2-19944]

Immunocytochemistry/ Immunofluorescence: Prohibitin Antibody [NBP2-19944]

Immunocytochemistry/Immunofluorescence: Prohibitin Antibody [NBP2-19944] - SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37 c for 30 min.Green: Prohibitin protein stained by Prohibitin antibody diluted at 1:1000.Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944]

Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944]

Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP2-19944] - Paraffin-embedded mouse liver. Prohibitin antibody dilution: 1:500.
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Applications for Prohibitin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Prohibitin

Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq]

Alternate Names

PHB

Gene Symbol

PHB1

UniProt

P35232

Additional Prohibitin Products

Product Documents for Prohibitin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Prohibitin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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