Prolactin R Antibody (B6.2 + PRLR742)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34286
![Immunohistochemistry-Paraffin: Prolactin R Antibody (B6.2 + PRLR742) [NBP2-34286]](https://resources.bio-techne.com/images/products/Prolactin-R-Antibody-B6-2-+-PRLR742-Immunohistochemistry-Paraffin-NBP2-34286-img0001.jpg "Immunohistochemistry-Paraffin: Prolactin R Antibody (B6.2 + PRLR742) [NBP2-34286]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Kappa/IgG1 Kappa Clone # B6.2 + PRLR742
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Semi-Purified human Prolactin R (B6.2); Recombinant human Prolactin R (PRLR742) (Uniprot: P16471)
Localization
Cell surface and cytoplasmic
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 Kappa/IgG1 Kappa
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-34555)
Antibody with azide - store at 2 to 8C.
Antibody with azide - store at 2 to 8C.
Scientific Data Images for Prolactin R Antibody (B6.2 + PRLR742)
Immunohistochemistry-Paraffin: Prolactin R Antibody (B6.2 + PRLR742) [NBP2-34286]
Immunohistochemistry-Paraffin: Prolactin R Antibody (B6.2 + PRLR742) [NBP2-34286] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Prolactin Receptor Monoclonal Antibody (B6.2 + PRLR742).Applications for Prolactin R Antibody (B6.2 + PRLR742)
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
0.25-0.5ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Prolactin R
Additional Prolactin R Products
Product Documents for Prolactin R Antibody (B6.2 + PRLR742)
Product Specific Notices for Prolactin R Antibody (B6.2 + PRLR742)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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