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Prolyl Oligopeptidase/PREP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-40792

Novus Biologicals, part of Bio-Techne
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NB100-40792

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-QYIVGRSRKQSNP corresponding to internal region according to NP_002717.3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Prolyl Oligopeptidase/PREP Antibody

Immunohistochemistry-Paraffin: Prolyl Oligopeptidase/PREP Antibody [NB100-40792]

Immunohistochemistry-Paraffin: Prolyl Oligopeptidase/PREP Antibody [NB100-40792]

Immunohistochemistry-Paraffin: Prolyl Oligopeptidase/PREP Antibody [NB100-40792] - Staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Prolyl Oligopeptidase/PREP Antibody [NB100-40792]

Immunohistochemistry-Paraffin: Prolyl Oligopeptidase/PREP Antibody [NB100-40792]

Immunohistochemistry-Paraffin: Prolyl Oligopeptidase/PREP Antibody [NB100-40792] - staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Prolyl Oligopeptidase/PREP Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:16000
Application Notes
WB: Preliminary experiments gave an approx. 45 kDa band in human brain, lymph node, spleen and testis lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 80.7 kDa band according to NP_002717.3. The 45 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows distinct staining patterns in the cytoplam of PCT.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Prolyl Oligopeptidase/PREP

The protein encoded by the Prolyl Endopeptidase gene is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides. (provided by RefSeq)

Alternate Names

PE, PREP, rPop

Entrez Gene IDs

5550 (Human); 19072 (Mouse); 83471 (Rat)

Gene Symbol

PREP

Additional Prolyl Oligopeptidase/PREP Products

Product Documents for Prolyl Oligopeptidase/PREP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Prolyl Oligopeptidase/PREP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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