Proprotein Convertase 9/PCSK9 Antibody (7F2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26436
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7F2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Proprotein Convertase 9/PCSK9 Antibody (7F2)
Immunogen
A synthesized peptide derived from Human Proprotein Convertase 9/PCSK9 [UniProt Q8NBP7]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Proprotein Convertase 9/PCSK9 Antibody (7F2)
Immunohistochemistry: Proprotein Convertase 9/PCSK9 Antibody (7F2) [NBP3-26436] -
Immunohistochemistry: Proprotein Convertase 9/PCSK9 Antibody (7F2) [NBP3-26436] - Image of Proprotein Convertase 9/PCSK9 Antibody (7F2) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: Proprotein Convertase 9/PCSK9 Antibody (7F2) [NBP3-26436] -
Western Blot: Proprotein Convertase 9/PCSK9 Antibody (7F2) [NBP3-26436] - Positive Western Blot detected in: PC-3 whole cell lysate.All lanes: Proprotein Convertase 9/PCSK9 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 75, 21 kDa
Observed band size: 60, 75 kDa
Applications for Proprotein Convertase 9/PCSK9 Antibody (7F2)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Proprotein Convertase 9/PCSK9
Alternate Names
FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, PCSK9
Gene Symbol
PCSK9
Additional Proprotein Convertase 9/PCSK9 Products
- All Products for Proprotein Convertase 9/PCSK9
- Proprotein Convertase 9/PCSK9 cDNA Clones
- Proprotein Convertase 9/PCSK9 ELISA Kits
- Proprotein Convertase 9/PCSK9 Lysates
- Proprotein Convertase 9/PCSK9 Primary Antibodies
- Proprotein Convertase 9/PCSK9 Proteins and Enzymes
- Proprotein Convertase 9/PCSK9 Simple Plex
Product Documents for Proprotein Convertase 9/PCSK9 Antibody (7F2)
Product Specific Notices for Proprotein Convertase 9/PCSK9 Antibody (7F2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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