Proprotein Convertase 9/PCSK9 Antibody (7F2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26436

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7F2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Proprotein Convertase 9/PCSK9 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Proprotein Convertase 9/PCSK9 [UniProt Q8NBP7]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Proprotein Convertase 9/PCSK9 Antibody (7F2)

Proprotein Convertase 9/PCSK9 Antibody (7F2)

Immunohistochemistry: Proprotein Convertase 9/PCSK9 Antibody (7F2) [NBP3-26436] -

Immunohistochemistry: Proprotein Convertase 9/PCSK9 Antibody (7F2) [NBP3-26436] - Image of Proprotein Convertase 9/PCSK9 Antibody (7F2) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Proprotein Convertase 9/PCSK9 Antibody (7F2)

Western Blot: Proprotein Convertase 9/PCSK9 Antibody (7F2) [NBP3-26436] -

Western Blot: Proprotein Convertase 9/PCSK9 Antibody (7F2) [NBP3-26436] - Positive Western Blot detected in: PC-3 whole cell lysate.
All lanes: Proprotein Convertase 9/PCSK9 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 75, 21 kDa
Observed band size: 60, 75 kDa

Applications for Proprotein Convertase 9/PCSK9 Antibody (7F2)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Proprotein Convertase 9/PCSK9

PCSK9 encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. The encoded protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. This protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a third form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

Alternate Names

FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, PCSK9

Gene Symbol

PCSK9

Additional Proprotein Convertase 9/PCSK9 Products

Product Documents for Proprotein Convertase 9/PCSK9 Antibody (7F2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Proprotein Convertase 9/PCSK9 Antibody (7F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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