Prostatic Acid Phosphatase/ACPP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51946

Novus Biologicals, part of Bio-Techne
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NBP1-51946
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Prostatic Acid Phosphatase/ACPP Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-YGIHKQKEKSRLQ corresponding to internal region according to NP_001090.2.

Epitope

C-YGIHKQKEKSRLQ

Specificity

This antibody is expected to recognize both isoforms (NP_001090.2; NP_001127666.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Prostatic Acid Phosphatase/ACPP Antibody

Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946]

Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946]

Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946] - Staining of Human Prostate lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946]

Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946]

Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-51946] - Staining of paraffin embedded Human Prostate with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Prostatic Acid Phosphatase/ACPP Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 5 ug/mL

Immunohistochemistry-Paraffin

3 - 5 ug/mL

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1 ug/mL
Application Notes
WB: Approx. 50 kDa band observed in human prostate lysates is reported in scientific literature (PMID: 3530530). IHC-P: Human prostate shows cytoplasm staining in some secretory cells of the gland.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Prostatic Acid Phosphatase/ACPP

Prostatic acid phosphatase is one of the two antigenic markers of prostatic carcinoma, the other being prostate specific antigen. It belongs to the kallikrein family of serine proteases and is suggested to act as a hydrolase to split phospharyl choline in semen and as a transferase. This antibody does not inhibit the enzymatic activity of PSAP. It reacts with non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no cross reaction with other phosphatases.

Alternate Names

ACP3, ACPP

Entrez Gene IDs

55 (Human); 56318 (Mouse); 56780 (Rat)

Gene Symbol

ACP3

UniProt

NP_001090.2 (Human)

Additional Prostatic Acid Phosphatase/ACPP Products

Product Documents for Prostatic Acid Phosphatase/ACPP Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Prostatic Acid Phosphatase/ACPP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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