Prostatic Acid Phosphatase/ACPP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78280
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-NHMKRATQIPSYKK corresponding to internal region according to NP_001127666.1, NP_001090.2.
Epitope
C-NHMKRATQIPSYKK,
Specificity
This antibody is expected to recognize both reported isoforms (NP_001127666.1; NP_001090.2).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Prostatic Acid Phosphatase/ACPP Antibody
![Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-78280]](https://resources.bio-techne.com/images/products/Prostatic-Acid-Phosphatase-ACPP-Antibody-Western-Blot-NBP1-78280-img0006.jpg "Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-78280]")
Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-78280]
Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-78280] - Staining of Human Prostate lysate with antibody at 0.01 ug/mL (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-78280]](https://resources.bio-techne.com/images/products/Prostatic-Acid-Phosphatase-ACPP-Antibody-Immunohistochemistry-Paraffin-NBP1-78280-img0005.jpg "Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-78280]")
Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-78280]
Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-78280] - Staining of paraffin embedded Human Prostate with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for Prostatic Acid Phosphatase/ACPP Antibody
Application
Recommended Usage
Immunohistochemistry
3 - 5 ug/mL
Immunohistochemistry-Paraffin
3 - 5 ug/mL
Peptide ELISA
Detection limit 1:32000
Western Blot
0.01 - 0.03 ug/mL
Application Notes
WB: Approx. 50 kDa band observed in human prostate lysates (calculated MW of 48.3 kDa band according to NP_001127666.1). IHC-P: Human prostate shows reticulate cytoplasm staining, more intens towards the lumen of the gland.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Prostatic Acid Phosphatase/ACPP
Additional Prostatic Acid Phosphatase/ACPP Products
Product Documents for Prostatic Acid Phosphatase/ACPP Antibody
Product Specific Notices for Prostatic Acid Phosphatase/ACPP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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