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Proteasome 19S S3 Antibody (OTI2A2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01808

Novus Biologicals, part of Bio-Techne
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NBP2-01808
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2A2

Concentration

0.95 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human PSMD3(NP_002800) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

60.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Proteasome 19S S3 Antibody (OTI2A2)

Western Blot: Proteasome 19S S3 Antibody (2A2) [NBP2-01808] Analysis of extracts (35ug) from 9 different cell lines by using anti-Proteasome 19S S3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Flow Cytometry: Proteasome 19S S3 Antibody (OTI2A2) [NBP2-01808]

Flow Cytometry: Proteasome 19S S3 Antibody (OTI2A2) [NBP2-01808]

Flow Cytometry: Proteasome 19S S3 Antibody (2A2) [NBP2-01808] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Proteasome 19S S3 antibody, and then analyzed by flow cytometry.
Western Blot: Proteasome 19S S3 Antibody (OTI2A2) [NBP2-01808]

Western Blot: Proteasome 19S S3 Antibody (OTI2A2) [NBP2-01808]

Western Blot: Proteasome 19S S3 Antibody (2A2) [NBP2-01808] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S S3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 19S S3.

Applications for Proteasome 19S S3 Antibody (OTI2A2)

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.95 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Proteasome 19S S3

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. [provided by RefSeq].

Alternate Names

26S proteasome regulatory subunit RPN3, 26S proteasome regulatory subunit S3, P58, proteasome (prosome, macropain) 26S subunit, non-ATPase, 3, Proteasome subunit p58, Rpn3, S3, 26S proteasome non-ATPase regulatory subunit 3, tissue specific transplantation antigen 2, TSTA2

Entrez Gene IDs

5709 (Human)

Gene Symbol

PSMD3

Additional Proteasome 19S S3 Products

Product Documents for Proteasome 19S S3 Antibody (OTI2A2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Proteasome 19S S3 Antibody (OTI2A2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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