Proteasome 20S alpha 3 Antibody (7X8W9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16551

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16551-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7X8W9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Proteasome 20S alpha 3 Antibodies »

Product Summary for Proteasome 20S alpha 3 Antibody (7X8W9)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 21-150 of human Proteasome 20S alpha 3 (P25788). VFQVEYAMKAVENSSTAIGIRCKDGVVFGVEKLVLSKLYEEGSNKRLFNVDRHVGMAVAGLLADARSLADIAREEASNFRSNFGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGSYSVNDGAQLY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Proteasome 20S alpha 3 Antibody (7X8W9)

Western Blot: Proteasome 20S alpha 3 Antibody (7X8W9) [NBP3-16551]

Western Blot: Proteasome 20S alpha 3 Antibody (7X8W9) [NBP3-16551]

Western Blot: Proteasome 20S alpha 3 Antibody (7X8W9) [NBP3-16551] - Western blot analysis of extracts of various cell lines, using Proteasome 20S alpha 3 Rabbit mAb (NBP3-16551) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 3 Antibody (7X8W9) [NBP3-16551]

Immunohistochemistry-Paraffin: Proteasome 20S alpha 3 Antibody (7X8W9) [NBP3-16551]

Immunohistochemistry-Paraffin: Proteasome 20S alpha 3 Antibody (7X8W9) [NBP3-16551] - Immunohistochemistry of paraffin-embedded mouse kidney using Proteasome 20S alpha 3 Rabbit mAb (NBP3-16551) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 3 Antibody (7X8W9) [NBP3-16551]

Immunohistochemistry-Paraffin: Proteasome 20S alpha 3 Antibody (7X8W9) [NBP3-16551]

Immunohistochemistry-Paraffin: Proteasome 20S alpha 3 Antibody (7X8W9) [NBP3-16551] - Immunohistochemistry of paraffin-embedded rat ovary using Proteasome 20S alpha 3 Rabbit mAb (NBP3-16551) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for Proteasome 20S alpha 3 Antibody (7X8W9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:1000 - 1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Proteasome 20S alpha 3

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. (provided by RefSeq)

Long Name

Proteasome subunit alpha type-3

Alternate Names

HC8, PSC8, PSMA3

Gene Symbol

PSMA3

Additional Proteasome 20S alpha 3 Products

Product Documents for Proteasome 20S alpha 3 Antibody (7X8W9)

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Certificate of Analysis

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Product Specific Notices for Proteasome 20S alpha 3 Antibody (7X8W9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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