Proteasome 26S S5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87800

Novus Biologicals, part of Bio-Techne
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NBP1-87800
NBP1-87800-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Proteasome 26S S5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AAQALALLREVARLEAPLEELRALHSVLQAVPLNELRQQAAELRLGPLFSLLNENHREKTTLCVSILERLLQAMEPVHVARNLRVDLQRGLIHPDDSVKILTLSQIGRIVENSDAVTEILNNAELLKQIVYCIGGENLS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Proteasome 26S S5 Antibody

Western Blot: Proteasome 26S S5 Antibody [NBP1-87800]

Western Blot: Proteasome 26S S5 Antibody [NBP1-87800]

Western Blot: Proteasome 26S S5 Antibody [NBP1-87800] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: Proteasome 26S S5 Antibody [NBP1-87800]

Immunocytochemistry/ Immunofluorescence: Proteasome 26S S5 Antibody [NBP1-87800]

Immunocytochemistry/Immunofluorescence: Proteasome 26S S5 Antibody [NBP1-87800] - Staining of human cell line A-431 shows localization to nucleus & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Proteasome 26S S5 Antibody [NBP1-87800]

Immunohistochemistry-Paraffin: Proteasome 26S S5 Antibody [NBP1-87800]

Immunohistochemistry-Paraffin: Proteasome 26S S5 Antibody [NBP1-87800] - Staining of human prostate shows strong nuclear positivity in glandular cells.
View 1 more images

Applications for Proteasome 26S S5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Proteasome 26S S5

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator base.

Alternate Names

KIAA007226S proteasome non-ATPase regulatory subunit 5, proteasome (prosome, macropain) 26S subunit, non-ATPase, 5,26S proteasome subunit S5B, S5BMGC23145,26S protease subunit S5 basic

Gene Symbol

PSMD5

Additional Proteasome 26S S5 Products

Product Documents for Proteasome 26S S5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Proteasome 26S S5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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