Proteasome beta 1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93490

Novus Biologicals, part of Bio-Techne
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NBP2-93490-0.02ml
NBP2-93490-0.1ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Proteasome beta 1 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 29-241 of human PSMB1 (NP_002784.1). RFSPYVFNGGTILAIAGEDFAIVASDTRLSEGFSIHTRDSPKCYKLTDKTVIGCSGFHGDCLTLTKIIEARLKMYKHSNNKAMTTGAIAAMLSTILYSRRFFPYYVYNIIGGLDEEGKGAVYSFDPVGSYQRDSFKAGGSASAMLQPLLDNQVGFKNMQNVEHVPLSLDRAMRLVKDVFISAAERDVYTGDALRICIVTKEGIREETVSLRKD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Proteasome beta 1 Antibody - BSA Free

Western Blot: Proteasome beta 1 AntibodyBSA Free [NBP2-93490]

Western Blot: Proteasome beta 1 AntibodyBSA Free [NBP2-93490]

Western Blot: Proteasome beta 1 Antibody [NBP2-93490] - Analysis of extracts of various cell lines, using Proteasome beta 1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: Proteasome beta 1 Antibody - BSA Free [NBP2-93490]

Immunocytochemistry/ Immunofluorescence: Proteasome beta 1 Antibody - BSA Free [NBP2-93490]

Immunocytochemistry/Immunofluorescence: Proteasome beta 1 Antibody [NBP2-93490] - Analysis of U-2 OS cells using Proteasome beta 1 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: Proteasome beta 1 Antibody - BSA Free [NBP2-93490]

Immunocytochemistry/ Immunofluorescence: Proteasome beta 1 Antibody - BSA Free [NBP2-93490]

Immunocytochemistry/Immunofluorescence: Proteasome beta 1 Antibody [NBP2-93490] - Analysis of C6 cells using Proteasome beta 1 at dilution of 1:100. Blue: DAPI for nuclear staining.
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Applications for Proteasome beta 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Proteasome beta 1

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species. [provided by RefSeq]

Long Name

Proteasome subunit beta type-1

Alternate Names

PSC5, PSMB1

Gene Symbol

PSMB1

Additional Proteasome beta 1 Products

Product Documents for Proteasome beta 1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Proteasome beta 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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